Immunofluorescence staining for the diagnosis of herpes encephalitis

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Immunofluorescence staining for the diagnosis of herpes encephalitis.

Direct immunofluorescence staining for herpes simplex virus was applied to cryostat sections of 43 specimens removed at brain biopsy. Herpes antigen was found in 10 specimens and virus was isolated from them. Antigen was found in one specimen from which virus was not isolated. Two specimens from which virus was isolated gave equivocal fluorescence. Thirty specimens gave no fluorescence or live ...

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Non-invasive method for early diagnosis of herpes simplex encephalitis.

For the early diagnosis of herpes simplex encephalitis IgG and IgM antibodies to herpes simplex virus in cerebrospinal fluid were measured by an enzyme linked immunosorbent assay (ELISA) and a local production index was calculated. Using these three criteria, 31 cases of various neurological illnesses were analysed. All eight cases of herpes simplex encephalitis were diagnosed correctly in the ...

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Immunofluorescence staining for detection of variola virus.

In September 1975 Bangladesh was the only country in the world with endemic variola major, and the eradication of the disease was imminent. A rapid and accurate laboratory diagnostic method was required to supplement immunodiffusion in agar gel and culture on chorioallantoic membrane of embryonated egg available at the Institute of Public Health in Dacca, Bangladesh. To determine its effectiven...

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Diagnosis of herpes virus hominis keratitis by immunofluorescence.

The high prevalence and destructive effect on the human cornea of herpes virus hominis (HVH) makes its detection desirable atan early stage in eye involvement (Duke-Elder, I965) . The action of the virus in the human cornea, at least when associated with active corneal disease, can produce a keratopathy with certain well-known clinical signs. The presence of one or more of these clinical signs ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1974

ISSN: 0021-9746

DOI: 10.1136/jcp.27.6.495